Avian flavivirus enters bhk-21 cells by low ph-dependent endosomal pathway

HIGHLIGHTS

• who: Duck Tembusu virus et al. from the Dulbecco's modified essential medium (DMEM, GIBCO, Invitrogen, Carlsbad, CA, USA) supplemented with, % fetal bovine serum (FBS, GIBCO, Grand island, NY, USA), µg/mL streptomycin, and , IU/mL penicillin (GIBCO, Grand island, NY, USA) was used for the baby hamster kidney , (BHK-21, ATCC CCL-10) cell culture, at , ◦ C in a, % , incubatorThe Duck Tembusu virus DTMUV strain XZ-2012, provided by Prof. Ruibing Cao, Nanjing Agricultural University (Genbank accession number: KM188953) was cultured in , cells supplemented with, % FBS. Two to three days later, following freezing and thawing three . . .
  

   

  

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