HIGHLIGHTS
SUMMARY
The amplification cycle was the first denaturation at 95 ◦ C for five minutes, 35 cycles of denaturation at 92 ◦ C for 45 s, annealing at 60 ◦ C for 45 s, extension at 72 ◦ C for 90 s, and a final extension step at 72 ◦ C for 10 min. After centrifugation at 1000 rcf for 60 s, the plate was incubated in a thermocycler: one cycle at 96 ◦ C for 60 s, 60 cycles of 96 ◦ C for 10 s, 50 ◦ C for five seconds and 60 ◦ C for four minutes. The amplification cycle was . . .
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