Bicistronic expression of a high-performance calcium indicator and opsin for all-optical stimulation and imaging at cellular resolution

HIGHLIGHTS

  • who: Paul K. LaFosse and colleagues from the Institutes of Health-University of Maryland Graduate Partnerships, Bethesda, MD, Neuroscience and have published the paper: Bicistronic Expression of a High-Performance Calcium Indicator and Opsin for All-Optical Stimulation and Imaging at Cellular Resolution, in the Journal: (JOURNAL) of September/12,/2022
  • what: To address these issues, here the authors demonstrate a single Cre-dependent virus that expresses both opsin and indicator in transfected cells without requiring multiple overlapping viral injections. The authors report average stimulation power over the milliseconds-long stimulation pulses: that is, the . . .

     

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