Binding with heat shock cognate protein hsc70 fine-tunes the golgi association of the small gtpase arl5b

HIGHLIGHTS

• who: Ebsy Jaimon from the C, % CO Constructs and antibodies pGEX, GFP-Nanobody was purchased from Addgene (#61838, deposited by Kazuhisa Nakayama). ARL, GFP was cloned in pLVX-TRE G vector (Takara) for mammalian expression and in pET28a vector for bacterial expression using the ARL, GFP construct as a template (a kind gift from Prof. Sean Munro, MRC Laboratory of Molecular Biology). ARL BQ, GFP and ARL BT, GFP were kind gifts from Prof. Paul Gleeson (The University of Melbourne). All mutants of ARL B were generated by site-directed mutagenesis using pET28a-ARL B as a . . .
  

   

  

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