Bioenergetics of pollen tube growth in arabidopsis thaliana revealed by ratiometric genetically encoded biosensors

HIGHLIGHTS

• who: Jinhong Liu from the Plasmid construction and generation of transgenic plant lines To construct the plant transformation vectors carrying the secondgeneration biosensors, the iNAPs and SoNar cDNAs were amplified from the first-generation pENTR- iNAP1, pENTR-iNAP4, and pENTRSoNar vectors, with a , bp overhang forward primer carrying a Bam, restriction site, whereas the mCherry gene was amplified from a pENTR-mCherry-, vector with a , bp overhang reverse primer carrying a BamHI restriction siteThis, aa peptide linker (GGSGG), was created via the overhang of the forward and reverse primer from SoNar/iNAPs and mCherry, respectively. The mCherry . . .
  

   

  

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