HIGHLIGHTS
SUMMARY
NIR fluorescent probes offer deeper light penetration and lesser tissue autofluorescence, giving rise to higher signal-to-background ratios for in_vivo imaging9-14. Bioorthogonal chemistry has emerged as a promising tool for designing smart NIR optical probes for sensitive in_vivo imaging due to high selectivity, rapid reaction rates, and good biocompatibility22-25. In recent years, bioorthogonal chemistry has seen widespread applications in optical imaging such as visualizing dynamic processes of endogenous molecules (i.e., enzymes, lipids, RNA, etc.)23,26-29, pre-targeted imaging of disease biomarkers30-33, in situ metabolic labeling and imaging . . .
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