Bot-4-one attenuates nlrp3 inflammasome activation: nlrp3 alkylation leading to the regulation of its atpase activity and ubiquitination

HIGHLIGHTS

• who: Do-Wan Shim from the University of College of Medicine, South Korea) Penicillin-streptomycin, fetal bovine serum (FBS), opti-MEM, and RPMI , were purchased from Gibco (Grand Island, NY, USA). IL-1β antibody (AF401), and IL-1β enzyme-linked immunosorbent assay (ELISA) kit were purchased from RandD Systems (Minneapolis, MN, USA). Antibodies against ASC (AL177)8, (Cryo-2)8, and caspase, (clone Casper-1), were purchased from Adipogen (San Diego, CA, USA). β-actin (sc-1616), and ubiquitin (sc-8017), were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monosodium urate (MSU) crystal, Bay11-7082, silica . . .
  

   

  

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