Brain delivery of igf1r5, a single-domain antibody targeting insulin-like growth factor-1 receptor

HIGHLIGHTS

• who: Alvaro Yogi et al. from the The nanoflow ultrahigh performance liquid chromatography-coupled selected-reaction monitoring (nanoLC-SRM) mass spectrometry method was used to quantify absolute or relative levels of proteins in a BBB model, serum, cerebrospinal fluid and vessel-depleted brain parenchyma fractionsAll protein extracts were reduced, alkylated and trypsin-digested using a previously described protocol [28]. Mass spectrometry analyses were carried out on a NanoAcquity UPLC (Waters, Milford, MA, USA) containing a , PepMapu2122 , trap (ThermoFisher, Waltham, MA, USA) followed by a nanoLC BEH , column (Waters) coupled with ESI-LTQ-XL-ETD or ESI-TSQ . . .
  

   

  

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