Brb-seq: ultra-affordable high-throughput transcriptomics enabled by bulk rna barcoding and sequencing

HIGHLIGHTS

• who: Daniel Alpern from the Total RNA was isolated using TRI Reagent (Molecular Research Center, #TR118) followed by double precipitation with ethanolThe RNA concentration was determined using the Qubit RNA HS Assay Kit (Invitrogen, #Q32852), and integrity was assessed using a Fragment Analyzer (Advanced Analytical). The RNA from each differentiation time point was used in two technical replicates, resulting in four samples pooled per library. Libraries were prepared with the BRB-seq protocol using total RNA amounts ranging from , ng to , μg per sample (Additional file, : Table, ). RNA fragmentation was done using the NEBNext Magnesium RNA Fragmentation . . .
  

   

  

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