HIGHLIGHTS
- who: Zhiyu Lin and colleagues from the School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, China have published the paper: Bridging PCR: An Efficient and Reliable Scheme Implemented for Genome-Walking, in the Journal: (JOURNAL)
- what: In this work, three walker primer sets were designed and individually paired with a SSP set to perform three parallel flank.
SUMMARY
Genome-walking is referred to a strategy used to retrieve unknown flanking sequences starting from a known DNA. Based on the involved rationales, these methods could be classified into inverse PCR, digestion . . .
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