Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens

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SUMMARY

    This protocol increased the astrocyte Ca2+ spike frequency in both the AcbC (1.62 ± 0.23 change from basal, P=0.02) and AcbSh (2.62 ± 0.52 change from basal, P=0.003) (2420 ROIs; n=25 slices, 8 mice; Fig 2H, right) without variations in the Ca2+ amplitude of the responses (Supplementary Fig S5A). Surprisingly, these Ca2+ dynamics in response to optostimulation did not show the same pattern of activity across NAc regions as observed by the glutamatergic inputs` innervation, indicating that astrocytes were not responding exclusively in areas within the direct . . .

     

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