Calcium plays a central role in the sensitization of trpv3 channel to repetitive stimulations*

HIGHLIGHTS

• who: Rui Xiao from the EXPERIMENTAL PROCEDURES DNA Constructs and Mutagenesis-Murine , cDNA in the pc, (Invitrogen) and pIRES EGFP (Clontech) vectors were obtained as described previously (15)To change Asp, to Asn, two oligonucleotides, ⬘-ATGTTCAGGTTACCCAGGCCTATGGTGAG and, ⬘-GGCCTGGGTAACCTGAACATCCAGCAG, were synthesized (Integrated DNA Technologies, Inc [, ]. Coralville, IA) and then paired with oligonucleotide, ⬘-TGTCCTCATCTGGGCCAC and the , primer, respectively, in separate PCRs using the wild type , as the template. All PCR conditions were standard as described before (22) using Pfu polymerase and an annealing temperature of , °C. One of the PCR products was digested with BsrGI/BstEII and the other . . .
  

   

  

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