Can allele-specific loop-mediated isothermal amplification be used for rapid detection of target-site herbicide resistance in lolium spp.?

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SUMMARY

    Herbicide resistance can be due to mutation(s) in the herbicide target gene conferring amino-acid changes in the protein and consequent inhibition of herbicide binding, the so-called target-site herbicide resistance (TSR). For each target mutation two sets of specific primers were designed, one primer set specific for the nucleotide which identifies the wild_type (WT, responsible for the susceptible, S, phenotype) and one primer set specific for the nucleotide which identifies the mutated (MUT, responsible for the resistant, R, phenotype). For the target mutation 197, the results were consistent in only one . . .

     

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