Catch me if you can: a biotinylated proteoliposome affinity assay for the investigation of assembly of the mexa-mexb-oprm efflux pump from pseudomonas aeruginosa

HIGHLIGHTS

  • who: Martin Picard from the Goethe University Frankfurt, Germany have published the paper: Catch me if you can: a biotinylated proteoliposome affinity assay for the investigation of assembly of the MexA-MexB-OprM efflux pump from Pseudomonas aeruginosa, in the Journal: (JOURNAL)
  • what: The liposomes used in the experiment shown (A) were enriched with biotinylated lipids to investigate the kinetics of the tripartite complex assembly in the context of transport. The authors show that the mutation within the proton relay pathway does not impair the assembly, which indicates that the efflux pump assembly does not . . .

     

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