Cbp-hsf2 structural and functional interplay in rubinstein-taybi neurodevelopmental disorder

HIGHLIGHTS

• who: Auru00e9lie Thonel from the Plasmid constructs The human HSF2-Snap (WT/mutants) were constructed from the HSF2-Myc (WT/mutants) plasmid after digestion of the inserts by EcoRI and KpnI and cloning into the EcoRI and EcoRV sites in frame with the, terminal Flag tag in pSNAPf plasmid using In-Fusion Kit (Clontech)The human HSF2-YFP was constructed by PCR and cloned into the XhoI and SalI sites in frame with the, terminal YFP tag in EGFp-, plasmid using In-Fusion Kit (Clontech). All PCR-amplified products for both plasmids were sequenced to exclude the . . .
  

   

  

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