Cdk8 and cdk19 act redundantly to control the cftr pathway in the intestinal epithelium

HIGHLIGHTS

• who: Susana Prieto and colleagues from the performed with , solution (cat#, ) at high temperature (95-100u00b0C) or for , solution (cat#, ) at high temperature (91u00b0C) for a period time that is suitable for each specific antibodyEndogenous peroxidases were blocked with Discovery Inhibitor (cat#, ). For , immunostaining, signal enhancement was performed using the rabbit antibody anti-goat (Vector Laboratories, cat#BA500, :2, ) for , min at, u00b0C then using DISCOVERY OmniMap anti-rabbit HRP detection Kit (cat#, ) according to the manufacturer`s instructions. The same kit was used for all other rabbit primary antibodies to amplify the signal. Slides were incubated . . .
  

   

  

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