Cdkn1c negatively regulates rna polymerase ii c-terminal domain phosphorylation in an e2f1-dependent manner*

HIGHLIGHTS

• who: Yihong Ma from the pGL2-E , (26), and pcDNA3-HA-E , (27)Transient transfections for protein expression utilized the calcium phosphate method: , cells growing in p, plates and , ␮g of each CMV expression vector (or empty control to a total of , ␮g). Transfected cells were collected , h post transfection and were processed for Western blots and immunoprecipitation assays as described below. A shRNAi vector targeting CDKN C (TRCN0000039678) was obtained from Open Biosystems and was used as previously described (24). Pools of CDKN, deficient cells were created by transecting the CDKN C shRNAi vector into HeLa . . .
  

   

  

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