Cell-free measurements of brightness of fluorescently labeled antibodies

HIGHLIGHTS

  • who: Haiying Zhou from the DepartmentWashington University have published the research work: Cell-free measurements of brightness of fluorescently labeled antibodies, in the Journal: Scientific Reports Scientific Reports
  • what: The authors propose a rapid method to assess the fluorescence activity of labelled antibodies using a mix of polystyrene microspheres that are coated for high affinity to kappa chains (positive beads) and uncoated beads that do not bind antibodies (negative beads), but provide a measure of background fluorescence.
  • how: In the Stain Index (SI) measurements the fluorophore conjugated to an antibody is tested in . . .

     

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