HIGHLIGHTS
- who: Bastian Vu00f6geli from the Center for Northwestern University have published the research: Cell-free prototyping enables implementation of optimized reverse u03b2-oxidation pathways in heterotrophic and autotrophic bacteria, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
- what: The authors develop an automated liquid-handling workflow, allowing the authors to screen 440 unique enzyme combinations and 322 assay conditions. The authors show that the source strain used for iPROBE extracts influences the yields and specificity of the screened pathways and use previous in_vivo optimizations in E. coli to find an optimal strain for r-BOX cell . . .
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