Cell lineage reconstruction of early zebrafish embryos using label-free nonlinear microscopy

HIGHLIGHTS

• who: Nicolas Olivier and collaborators from the Although classical developmental biology is characterized by qualitative descriptions, recent work underlines the requirements for precise measurements to enable formal reconstruction integrating the genetic, molecular, and cellular levels of organization (1-3)The optimization of microscopy imaging techniques and improved data algorithmic processing are key issues in such reconstructions. Parallelized linear microscopy such as light-sheet fluorescence mi-croscopy provides fast imaging but suffers from loss of information with depth (4). Point-scanning two-photon microscopy provides deeper imaging (5) but exhibits slower frame rate, compromising , Laboratory for Optics and . . .
  

   

  

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