Cell-specific bioorthogonal tagging of glycoproteins

HIGHLIGHTS

• who: Anna Cioce from the Cloning and expression of , and , p, plasmids containing , or , were purchased from DNASU Plasmid Repository (Arizona State University, Tempe, USA)The coding sequences in p, (Hs, for , and Hs, for GALK2) were cloned into BamHI/BglII-digested pTriEX with an, terminal GST-3c cleavage site and a, terminal FLAGtag by using the In-Fusion HD Cloning Kit (Takara, Tokyo, Japan) and the following primers: inf_GALK1_F/R or inf_GALK2_F/R. Reaction was run for , cycles using the following protocol: , u00b0C (, s), u00b0C (, s), u00b0C (, s), u00b0C (, min, return to step, ), u00b0C (, min). Sf . . .
  

   

  

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