Characterization of a defensin from the oyster crassostrea gigas

HIGHLIGHTS

• who: Yannick Gueguen from the EXPERIMENTAL PROCEDURES Animals and RT-PCR Amplification of Cg-def-Adult oysters, Cgigas, were purchased from a local oyster farm in Normandie (France) or Palavas (Gulf of Lion, France) and kept in seawater at , u00b0C. A cDNA encoding part of a putative defensin was identified by randomly sequencing clones from a C. gigas mantle edge cDNA library constructed in u242d-ZAP II (University of Caen). Specific primers were then used to isolate the full-length Cg-def cDNA from the same library. Briefly, the, u2b18 missing end of the transcript was identified . . .
  

   

  

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