Characterization of a fold in tango1 evolved from sh3 domains for the export of bulky cargos

HIGHLIGHTS

• who: Oliver Arnolds from the Constructs MOTH domains of human TALI (23-123), Otoraplin (18-128), and MIA (19-131), and , (30-139) from Drosophila melanogaster (dmTANGO1(30-139)) were expressed from codon-optimized sequences in a modified p, expression vector, in , pRep, Ecoli strain. Human , (21-131) (hs, (21-131)) and , (21-151) (hs, (21-151)) were expressed from codon-optimized sequences in a modified pET19b vector (provided by Matthias Lu00fcbben, PhD of the Department of Biophysics at the Ruhr University of Bochum) in BL21(DE3)RIL E. coli strain. The sequences of the used constructs . . .
  

   

  

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