Characterization of two 1,3-β-glucan-modifying enzymes from penicillium sumatraense reveals new insights into 1,3-β-glucan metabolism of fungal saprotrophs

HIGHLIGHTS

SUMMARY

Probably, 1,3-β-glucan acting enzymes evolved in two distinct categories: (i) 1,3-β-glucan degrading enzymes, to degrade exogenous 1,3-β-glucan, and_(ii) 1,3-β-glucan modifying enzymes, to make endogenous 1,3-β-glucan more resistant against the degrading action of the first category of enzymes. Units are expressed as µmol reducing_ends (for PGA, CMC, Arabinoxylan, LAM5ol and laminarin substrates) and µmol p-nitrophenol (for pNPGLc, pNPGal and pNPLAM2 substrates) released per minute. To ascertain whether G9376 catalyzes 1,3-β-glucan hydrolysis with an exo or endo mode of action, the authors . . .

 



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