Charged multivesicular body protein 2b (chmp2b) of the endosomal sorting complex required for transport-iii (escrt-iii) polymerizes into helical structures deforming the plasma membrane*

HIGHLIGHTS

• who: Gilles Bodon from the EXPERIMENTAL PROCEDURES DNA Construct The wild-type human CHMPs ( A, A, B, and C) cDNAs were cloned from HeLa cells by RT-PCR, in frame with a, terminal FLAG inserted into reverse primers (supplemental Table, )CHMP B, CHMP Bintron5-FLAG, and CHMP, FLAG, CHMP BL D/L, FLAG and CHMP BL D/F, FLAG were generated by PCR using plasmids containing HA-CHMP B or GFP-VPS B as templates, introducing Stop codon or point mutations inside primers where needed. (All PCR primers used in this study are described in supplemental Table . . .
  

   

  

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