HIGHLIGHTS
- who: Benjamin C. Campbell from the (UNIVERSITY) have published the Article: Chemically stable fluorescent proteins for advanced microscopy, in the Journal: (JOURNAL) of 23/01/2022
- what: The authors report the rational engineering of a remarkably stable yellow fluorescent protein (YFP), u2018hyperfolder YFP` (hfYFP), that withstands chaotropic conditions that denature most biological structures within seconds, including superfolder green fluorescent protein (GFP). hfYFP contains no cysteines, is chloride insensitive and tolerates aldehyde and osmium tetroxide fixation better than common fluorescent proteins, enabling its use in expansion and electron microscopies. The authors aimed to exploit the high . . .
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