Chick fetal organ spheroids as a model to study development and disease

HIGHLIGHTS

• who: Soran Dakhel from the Fertilized white Lohman and Bovan chicken eggs (Gallus gallus) were obtained from Stru00f6mbu00e4cks u00c4gg, Vu00e4nnu00e4s, Sweden, and incubated in a , u00b0C humidified egg incubatorThe embryos were harvested at embryonic day (E) , and, and staged according to the protocol of Hamburger and Hamilton [14]. Embryos were maintained on ice in phosphate-buffered saline (PBS, pH, .4) prior to organ dissection. The use of fertilized chick eggs prior to , does not require ethical permission. Experiments using chick embryos at , was approved by the Institutional Animal Care and Use Committee of Umeu00e5 University, Sweden (Dnr . . .
  

   

  

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