Chimeric dna byproducts in strand displacement amplification using the t7 replisome

HIGHLIGHTS

• who: Dillon B. Nye and Nathan A. Tanner from the (UNIVERSITY) have published the article: Chimeric DNA byproducts in strand displacement amplification using the T7 replisome, in the Journal: PLOS ONE of 19/08/2022
• what: The authors investigate strand-displacement amplification reactions using the T7 replisome a macromolecular complex of a helicase a single-stranded DNA binding protein and a DNA polymerase.
• future: Experiments are needed to evaluate the utility of T7 SDA in unbiased non-specific amplification for long- and short-read sequencing methods.

SUMMARY

Isothermal amplification . . .

 



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