Colorectal using algorithmic analysis of rna sequencing data

HIGHLIGHTS

• who: Sorokin et_al BMC Cancer and collaborators from the To isolate RNA, u00b5M, thick paraffin slices were trimmed from each FFPE tissue block using microtomeRNA was extracted from FFPE slices using QIAGEN RNeasy FFPE Kit following the manufacturer`s protocol. RNA , Nano or Qubit RNA Assay kits were used to measure RNA concentration. RNA Integrity Number (RIN) was measured using Agilent , bio-Analyzer. For depletion of ribosomal RNA and library construction, KAPA RNA Hyper with rRNA erase kit (HMR only) was used. Different adaptors were used for multiplexing samples in one sequencing run. Library concentrations and quality . . .
  

   

  

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