Colorimetric lpmo assay with direct implication for cellulolytic activity

HIGHLIGHTS

  • who: Søren Brander from the Department of Geosciences and Natural Resource Management, University of Copenhagen, Copenhagen, Denmark have published the article: Colorimetric LPMO assay with direct implication for cellulolytic activity, in the Journal: (JOURNAL)
  • what: The authors show that LPMOs can oxidise rPHP at neutral pH through a mechanism that is enhanced by dehydroascorbate (DHA). For this experiment, the TaAA9A catalysed oxidation of rPHP was studied in a combination of conditions with and without ascorbate, DHA, ­O2, and ­H2O2 (Fig 5a).
  • how: The disappearance of ascorbate was followed by measuring the specific . . .

     

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