Combinatorial crispr interference library for enhancing 2,3-bdo production and elucidating key genes in cyanobacteria

HIGHLIGHTS

  • who: PCC and collaborators from the Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, Department of Life Science, National have published the paper: Combinatorial CRISPR Interference Library for Enhancing 2,3-BDO Production and Elucidating Key Genes in Cyanobacteria, in the Journal: (JOURNAL)
  • how: The authors first confirmed that SpCas9 SaCas9 and St1Cas9 can be used as orthogonal CRISPR systems for genome engineering of PCC7942 . In contrast to the aforementioned strategies and owing to the ability of orthogonal SaCas9 to integrate the SpdCas9-based CRISPRi library for multiplex suppression the authors developed a . . .

     

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