Combined enhancement of the propionyl-coa metabolic pathway for efficient androstenedione production in mycolicibacterium neoaurum

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• who: Zhenhua Su from the All strains and plasmids used in this study are listed in Table, Ecoli DH5u03b1 was used for plasmid construction. Strain MNR was stored in Tianjin University of Science and Technology Culture Collection Center (TCCC), Tianjin, China, and used for constructing engineering strains. E.coli was cultured in an LB medium. The culture method and medium of MNR were performed as previously described [29]. The medium of recombinant Mycolicibacterium strains was supplemented with kanamycin (, u03bcg/mL). The p, plasmid was modified using primers to construct an expression regulatory plasmid have published the Article . . .
  

   

  

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