HIGHLIGHTS
- who: Aikaterini Geladaki from the Department University of have published the Article: Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
- what: The authors compare protein subcellular localisation maps produced by LOPITDC and hyperLOPIT using the human osteosarcoma U-2 OS cell line and evaluate the impact of employing a differential_centrifugation-based workflow on global, experiment-wide resolution. Instead, the majority of classification disparities between the LOPIT -DC and hyperLOPIT data stem from cases where a protein was assigned to a unique subcellular niche in one dataset . . .
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