HIGHLIGHTS
SUMMARY
The combination of in_vitro 3D models with in_vivo models might take preclinical research to the next step allowing to perform more robust and reliable research and, in turn, to develop more effective anti-cancer treatments. The authors will discuss the currently available strategies to improve and integrate in_vitro and in_vivo tumor models, to better understand the 2 Cellular composition (Who)? Today, wide varieties of in_vivo models are used to reconstruct primary and metastatic tumors niche and microenvironment. Conversely, current in_vitro models using immortalized cell lines, rather than primary cells, fail to recapitulate many of . . .
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