HIGHLIGHTS
SUMMARY
All colonies were maintained in plastic cases at 25 °C in constant darkness until the induction of the worker-worker, worker-presoldier, and nymph-nymphoid molts (see below). The 5th stage nymphs (N5 nymphs) and the 4th-5th stage workers (old-age workers) were collected from the colonies based on the shape of wing buds, body size, and antennal segments. To obtain individuals at multiple times during the worker-worker molt, the authors used the 20-hydroxyecdysone (20E) application method, as described previously23 (Fig 1A). As the method described for worker-worker molt ("Induction . . .
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