HIGHLIGHTS
SUMMARY
Strategies such as gene knockouts1, CRISPR mutations2, tissue-specific promoters3, and inducible and transcriptional activators4 have been developed to allow for temporal regulation of gene_expression. Conversely, in the absence of the molecular input, the activity of the ribozyme results in mRNA degradation and the suppression of gene_expression. Two of the switches that demonstrated turn-on activity in the presence of their respective target molecules, hypoxanthine and folinic acid, were then tested with pre-treatment of increasing concentrations of small_molecule under a range of incubation times post-transfection. Stock solutions of target small_molecules for cell . . .
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