Comprehensive analysis of disease pathology in immunocompetent and immunocompromised hosts following pulmonary sars-cov-2 infection

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  • who: Santhamani Ramasamy et al. from the Manassas, MA, USA) [14, ]Experiments involving infectious SARS-Co, were conducted in Biosafety level , facilities at Rutgers University, as per approved standard operating procedures. Unless specified, all chemicals and reagents were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA)., .2. SARS-Co, Infectivity Titration We performed a plaque assay to determine live viruses in the inoculum and tissue homogenates, as described previously [16]. The Vero , cell monolayer was infected with SARS-Co, using DMEM media (supplemented with, % FBS). The plaques were visualized on the, rd day by staining . . .

     

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