Comprehensive characteristics of pathological subtypes in testicular germ cell tumor: gene expression, mutation and alternative splicing

HIGHLIGHTS

SUMMARY

The authors comprehensively analyzed the differences in gene_expression, alternative splicing (AS), and somatic mutations between TGCT subtypes. Differential analysis of mRNA expression and alternative splicing One-way analysis of variance (ANOVA) of gene_expression in the three main types of TGCT (embryonal carcinoma, mixed germ cell tumor, and seminoma) revealed the most significant differences between embryonal carcinoma and seminoma. Quantification of gene_expression and alternative splicing Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, USA) for TGCT_cell lines. cDNA synthesis was obtained by reverse transcription PCR (RT-PCR) using 5×HiScript III RT SuperMix . . .

 



If you want to have access to all the content you need to log in!

Thanks :)

Username or Email

Password

Remember me

Lost your password?

If you don't have an account, you can create one here.