Comprehensive proteomic analysis of interphase and mitotic 14-3-3-binding proteins*

HIGHLIGHTS

• who: Sarah E.M. Meek from the Materials-The, /, peptide, RDLSWLDLEAN, was synthesized by DrM. Berne (Tufts University) and HPLC-purified by Dr. J. Gorka (Biomolecules Midwest). Precast SDS-PAGE gels used for MS analysis were from Bio-Rad, and the colloidal Coomassie protein stain was from Invitrogen. Vivaspin and Centricon centrifugal concentrators were from Vivascience and Amersham Biosciences, respectively. All other reagents were from Sigma or Fisher and were reagent grade. Antibodies, Western Blotting, and Immunoprecipitation-Antibodies against, (K19), glutathione, transferase (GST), Cdc B, Cdc C, PCTAIRE, PCTAIRE, (tuberin), nucleolin (C23), nucleophosmin, Chk1, histone deacetylase, and . . .
  

   

  

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