Confocal imaging of biomarkers at a single-cell resolution: quantifying `living` in 3d-printable engineered living material based on pluronic f-127 and yeast

HIGHLIGHTS

  • who: Bojan u017dunar from the Centre de Biophysique Molu00e9culaire (CBM), CNRS, UPR, University of Orlu00e9ans and INSERM, Orlu00e9ans, France have published the Article: Confocal imaging of biomarkers at a single-cell resolution: quantifying `living` in 3D-printable engineered living material based on Pluronic F-127 and yeast, in the Journal: (JOURNAL)
  • what: The authors demonstrate the methodology to assess cell-scaffold interactions by characterizing intact alive yeast cells in cross-linked F127-based hydrogels using genetically encoded ratiometric biosensors to measure intracellular ATP and cytosolic pH at a single-cell level through confocal imaging.
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