Conformational changes in the human cx43/gja1 gap junction channel visualized using cryo-em

HIGHLIGHTS

• who: Hyuk-Joon Lee from the Protein expression and purification of human Cx43-WT Cx43-WT expression plasmids were amplified in Escherichia coli DH5u03b1 strains, purified, mixed with linear polyethylenimine (PEI, Polysciences, Inc), and transfected into human embryonic kidney E (HEK E) cells (ATCC, CRL-10852)9, . When the HEK E suspension cells (, L) were grown at , u00b0C to a density of ~0., u00d7 , cells/ml in Ca2+free Dulbecco`s modified Eagle`s medium (DMEM) supplemented with, % fetal bovine serum, the mixtures of DNA and PEI were added to the cells. Dimethyl sulfoxide (Amresco) was added . . .
  

   

  

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