Conformational transitions and allosteric modulation in a heteromeric glycine receptor

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  • who: Eric Gibbs from the Cryo-EM sample preparation and imaging Graphene-oxide (GO) coated grids were prepared from Cu , /1., holey carbon grids (Quantifoil, Micro Tools) using the drop-cast method72. A, ., mg/mL GO solution was prepared from a stock of , mg/mL GO solution diluted in MilliQ water (Sigma Aldrich, ), followed by centrifugation at, u00d7g for , min to remove large GO aggregates. EM Grids were glow discharged at , mA for , s, and then, ., u03bcL of the GO solution was applied to the carbon-coated side for , min. The solution was gently blotted away with . . .

     

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