Considerations and practical implications of performing a phenotypic crispr/cas survival screen

HIGHLIGHTS

  • who: Ator Ashoti and collaborators from the Editor:, Hirosaki University Graduate The Netherlands have published the Article: Considerations and practical implications of performing a phenotypic CRISPR/Cas survival screen, in the Journal: PLOS ONE of 12/05/2021
  • what: Genome-wide CRISPR screen reveals large chromosomal truncations Using the DIE cell system, the authors aimed to identify modulators of DUX4 cytotoxicity by performing a genome-wide CRISPR/Cas9 knockout screen. The authors focused further on hits that emerged in at least 3 out of the 4 screens. The authors focused on rtTA3 transgene that binds . . .

     

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