Construction of a set of novel transposon vectors for efficient silencing of protein and lncrna genes via crispr interference

HIGHLIGHTS

  • who: Maria Czarnek from the Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Kraku00f3w, Gronostajowa, u2011, Kraku00f3w, Poland have published the research: Construction of a Set of Novel Transposon Vectors for Efficient Silencing of Protein and lncRNA Genes via CRISPR Interference, in the Journal: (JOURNAL)
  • what: The aim of the work was to generate a single vector for stable expression of both elements of CRISPRi system: dCas9-based repressor and sgRNA, using non-commercial resources available via Addgene repository.
  • how: As a scaffold for the vector the authors . . .

     

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