HIGHLIGHTS
- who: Yanqing Li et al. from the College of Food Science and Technology, Shanghai Ocean University, Shanghai, China have published the research work: Construction of Biocatalysts Using the P450 Scaffold for the Synthesis of Indigo from Indole, in the Journal: (JOURNAL)
- what: We have successfully transformed indole into indigo by employing a DFSM-facilitated P450BM3 peroxygenase system using hydrogen peroxide as an oxidant.
SUMMARY
The chemoselectivity of three double mutants, F87A/T268A, F87A/T268I, and F87G/T268A, was 46%, 42%, and 44%, respectively, indicating that the reduction of the amino_acid size . . .
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