Construction of mutant glucose oxidases with increased dye-mediated dehydrogenase activity

HIGHLIGHTS

• who: Yohei Horaguchi and colleagues from the Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture have published the Article: Construction of Mutant Glucose Oxidases with Increased Dye-Mediated Dehydrogenase Activity, in the Journal: (JOURNAL)
• what: The authors focused on two representative GOxs of which crystal structures have already been reported-Penicillium amagasakiense GOx (PDB ID; 1gpe) and Aspergillus niger GOx (PDB ID; 1cf3). The authors conducted mutagenesis analyses of GOxs to construct GOxs with reduced oxidase activity, which is the oxidative half reaction using oxygen as the electron_acceptor, and increased dehydrogenase activity . . .
  

   

  

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