Copy-paste mutagenesis: a method for large-scale alteration of viral genomes

HIGHLIGHTS

  • who: Jiajia Tang and colleagues from the Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany have published the article: Copy-Paste Mutagenesis: A Method for Large-Scale Alteration of Viral Genomes, in the Journal: (JOURNAL)
  • what: The authors demonstrate that this method can be used to generate chimeric strains of human cytomegalovirus (HCMV) the largest human DNA virus. The analysis of the sequence confirmed that the chimera had been constructed intendedand andthat thatthere therewere wereno no unwanted unwanted mutations. constructed asas intended mutations.

SUMMARY

    Although the first herpesvirus BAC . . .

     

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