Coregulation of neurite outgrowth and cell survival by amphoterin and s100 proteins through receptor for advanced glycation end products (rage) activation*

HIGHLIGHTS

• who: Henri J. Huttunen from the Cell Culture and Transfection-, mouse neuroblastoma cells and , rat glioma cells were cultured in Dulbecco's modified Eagle's medium supplemented with , mg/ml streptomycin, units/ml penicillin G, and, % fetal calf serum. , neuroblastoma cells were transfected with Fugene , reagent according to the manufacturer's instructions (Roche Molecular Biochemicals). To obtain stably transfected clones the cells were first incubated with, ., mg/ml , (Life Technologies, Inc.) for , weeks. Then single cell clones were picked, seeded on, well plates, and incubated in the presence of , for another , weeks. High expression clones were then . . .
  

   

  

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