Cpg sites associated with nrp1, nrxn2 and mir-29b-2 are hypomethylated in monocytes during ageing

HIGHLIGHTS

• who: Monocytes et al. from the The study is approved by Ethics Review Committee on Human Research of the University of TartuAll of the participants gave written informed consent. Peripheral blood was obtained from healthy donors of Estonian Genome Center of University of Tartu. Peripheral blood mononuclear cells (PBMC) were extracted using a FicollPaque (GE Healthcare) gradient centrifugation. CD14+ monocytes were extracted from PBMCs using microbeads (CD14+ #130-050-201) and AutoMACS technology (Miltenyi Biotec). The purity of monocyte cell population was analysed with FACSCalibur (BD Biosciences) using fluorescence conjugated antibodies against , and , (Miltenyi) to confirm the . . .
  

   

  

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