HIGHLIGHTS
- What: Utilizing the doxycycline (Dox)-inducible dCas9-VP64-p65-Rta (dCas9-VPR) for CRISPRa and Dox-inducible dCas9-KRAB for CRISPRi, the authors aimed to regulate the transcriptional activity of the miR-23a ~ 27a ~ 24-2 cluster in HepG2 cells (Fig 5A, F). The authors used CRISPR KO to assess the potential role and gene network of individual mature miR-23a/ miR-27a in HCC cells. Other studies suggest that miR-23a/miR-27a regulate apoptosis in HCC cells , but the analysis showed no significant change in apoptosis after miR-23a KO or miR-27a KO.
If you want to have access to all the content you need to log in!
Thanks :)
If you don't have an account, you can create one here.